Lysozyme (LYZ), also known as cytosolic muramidase or N-acetylmuramide glycohydrolase, is an alkaline enzyme that hydrolyzes mucopolysaccharides in pathogenic bacteria. It mainly breaks the β-1,4 glycosidic bond between N-acetylmuramide and N-acetylamino glucose in the bacterial cell wall, causing the cell wall insoluble mucopolysaccharide to break down into soluble glycopeptides, resulting in the escape of the cell wall rupture contents and lysis of the bacteria. LYZ can also directly bind to negatively charged viral proteins and form complex salts with DNA, RNA and deacylated proteins to inactivate viruses. Therefore, the LYZ has antibacterial, anti-inflammatory and antiviral effects. In human body, LYZ is present in neutrophils, monocytes and macrophages; it is also present in mucosal secretion and becomes one of the body surface defense factors.
Rice-derived recombinant human lysozyme, OsrhLYZ, is expressed in rice through DNA recombinant technology, then extracted and purified.
Appearance: white or slightly pink lyophilized powder
Specification: 1g, 10g, 100g
Purity: >95.0% (SDS-PAGE)
Bioactivity: ≥ 40,000 IU/mg
In order to verify the antibacterial and bactericidal effect of LYZ, LYZ and Propionibacterium acnes were mixed incubation in the culture medium. The specific results are shown in the table below:
The pictures of culture medium are showed as below:
In addition to the bactericidal effect on Propionibacterium acnes, the data of several literature studies, the lysozyme also have antibacterial effect on Staphylococcus aureus, Staphylococcus Surface, Propionibacterium, Escherichia coli, Serratia, and the minimum inhibitory concentration of human lysozyme is 0.001-0.5mg/ml.
MIC is the abbreviation of minimum inhibitory concentration. The MIC is an indicator to measure the antibacterial activity of antibacterial effect, referring to the lowest drug concentration that can inhibit the growth of pathogenic bacteria in the culture medium after 18 to 24 hours of in vitro culture of bacteria.
Lysozyme has been widely used in biopharmaceutical, food and cosmetic industries in recent years. The OsrhLYZ, a recombinnat human lysozyme derived from rice, is definitely an ideal raw material for making a non-additive, transparent system of preservatives, because it is completely non-animal source, without any skin irritation, colorless and tasteless.
In addition to being a preservative, the OsrhLYZ is also a great ingredient for acne and anti-inflammatory skin care products.