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01.How to recontitute the recombinant proteinase K powder?
Answer:
Recommend reconstitute recombinant proteinase K powder in sterile water or 20mM Tris-HCl (pH 7.4~8.0), 1mM CaCl2, 50% glycerol at 20mg/ml. Further dilutions can be made in another aqueous buffer.
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02.How to test the activity of recombinant proteinase K?
Answer:
The activity of recombinant proteinase K is tested by hemoglobin, and one unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 µmol tyrosine per min at 37°C, pH 7.5.
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03.Is the recombinant proteinase K DNase and RNase free?
Answer:
Yes, it is.
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04.What methods do you use to detect DNase and RNase?
Answer:
We detect DNase with λ-DNA electrophoresis method and detect RNase with RT-PCR.
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05.What is the concentration of recombinant proteinase K solution? Are there any protective agents added?
Answer:
The concentration of recombinant proteinase K solution is 20mg/mL, and calcium and glycerin are added as protective agents.
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06.What is the price of recombinant proteinase K?
Answer:
The price depends on demand quantity and order times. But we can provide gradient quotation sheet like 10g, 50g and 100g. Price details can be negotiated.
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07.What is the production capacity of recombinant proteinase K?
Answer:
The capacity of recombinant proteinase K is about 80kg per month.
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08.Apart from DNase and RNase, do you detect other nucleases?
Answer:
No, we do not. If customers have any requirement, we could do it.
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09.What is the shelf life of recombinant proteinase K? How long can it be stored at room temperature?
Answer:
The recombinant proteinase K is recommended stored at temperature 2-8°C for at least 24 months. We do not recommend to store it at room temperature.
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10.What are the application areas of the growth factors products?
Answer:
Cell culture media and cosmetics.