• 01.How to recontitute the recombinant proteinase K powder?

    Recommend reconstitute recombinant proteinase K powder in sterile water or 20mM Tris-HCl (pH 7.4~8.0), 1mM CaCl2, 50% glycerol at 20mg/ml.  Further dilutions can be made in another aqueous buffer.

  • 02.How to test the activity of recombinant proteinase K?

    The activity of recombinant proteinase K is tested by hemoglobin, and one unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 µmol tyrosine per min at 37°C, pH 7.5.

  • 03.Is the recombinant proteinase K DNase and RNase free?

    Yes, it is.

  • 04.What methods do you use to detect DNase and RNase?

    We detect DNase with λ-DNA electrophoresis method and detect RNase with RT-PCR.

  • 05.What is the concentration of recombinant proteinase K solution? Are there any protective agents added?

    The concentration of recombinant proteinase K solution is 20mg/mL, and calcium and glycerin are added as protective agents.

  • 06.What is the price of recombinant proteinase K?

    The price depends on demand quantity and order times. But we can provide gradient quotation sheet like 10g, 50g and 100g. Price details can be negotiated.

  • 07.What is the production capacity of recombinant proteinase K?

    The capacity of recombinant proteinase K is about 80kg per month.

  • 08.Apart from DNase and RNase, do you detect other nucleases?

    No, we do not. If customers have any requirement, we could do it.

  • 09.What is the shelf life of recombinant proteinase K? How long can it be stored at room temperature?

    The recombinant proteinase K is recommended stored at temperature 2-8°C for at least 24 months. We do not recommend to store it at room temperature.

  • 10.What are the application areas of the growth factors products?

    Cell culture media and cosmetics.


Wuhan Healthgen Biotechnology Corp.

Tel. +86 027 59301891 Ext 8015

Fax. +86 027 59403931

E-mail cjing@oryzogen.com

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